New Ph.D. students: Welcome to Zaynab Jaber and Uthama Edupuganti, Ph.D. students in the CUNY Biochemistry Ph.D. program, who've joined the lab in spring 2016!

Just out - photoreceptors: We've examined the generality of the signaling model we determined for the phototropin/AsLOV2 system (Harper et al. 2003). An analogous mechanism is used is by EL222, a LOV-HTH protein (Nash et al. 2011, Rivera-Cancel et al. 2012), and EL346, a LOV-HK kinase (Corrêa et al., 2013, Rivera-Cancel et al. 2014), to convert light into changes in DNA binding and enzymatic activities. We've taken advantage of this to develop new optogenetic tools (Motta-Mena et al. 2014), and think that similar strategies can be applied to other LOV proteins (Glantz et al. 2016).

Just out - bHLH/PAS: We've reported two small molecule approaches to inhibit the HIF transcription factors in living cells. One route exploits a large cavity within the HIF-2α PAS-B domain (Scheuermann et al. 2009, Key et al. 2009), providing a site for high-affinity ligands to trigger allosteric changes that disrupt HIF-2α/ARNT complexes (Scheuermann et al. 2013, Scheuermann et al. 2015). Another strategy targets all HIF isoforms (Guo et al. 2013, Guo et al. 2015) by disrupting interactions between the ARNT subunit and CCC coactivator proteins, including TACC3 (Partch and Gardner 2011).

for May 24, 2017, as found in AB Ward et al., Science 339 (2013): 913:

"Any experimental observation can in principle be converted into a restraint for building ever more complex (structural) models. The reach and impact of structural biology can thus be extended to a wider and more diverse audience. Using these new computational and bioinformatics approaches to collect and integrate diverse pieces of structural and experimental data, Humpty Dumpty can be put back together again."